Tick species from cattle in the Adama Region of Ethiopia and pathogens detected

Abstract Ticks will diminish productivity among farm animals and transmit zoonotic diseases. We conducted a study to identify tick species infesting slaughter bulls from Adama City screen them for tick-borne pathogens. In 2016, 291 ticks were collected 37 in Adama, which ready slaughter. identified morphologically. Total genomic DNA was extracted used test Rickettsia spp. with real-time PCR. Species identification done by phylogenetic analysis using sequencing that targeted the 23S-5S intergenic spacer region ompA genes. Four two genera, Amblyomma Rhipicephalus , identified. cohaerens dominant (n = 241, 82.8%), followed variegatum 22, 7.5%), pulchellus 19, 6.5%), decoloratus 9, 3.0%). Among all ticks, 32 (11%) positive 15 (5.2%) of these as R. africae comprising at least genetic clades, occurring A. 10) 5). The remainder -positive samples could not be amplified due low yield. Furthermore, another carried other pathogenic bacteria: Ehrlichia ruminantium 9; 3.1%) sp. 3; 1%) Rh. Anaplasma 1; 0.5%) Neoehrlichia mikurensis 2; 0.7%) cohaerens. All negative Bartonella spp., Babesia Theileria Hepatozoon reported first time E. ruminatium N. sp., Medically veterinarily important pathogens mostly detected These data are relevant One-health approach monitoring prevention disease transmission.